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Fig. 4 Fig. 4. Apoptosis analysis. Zebrafish larvae were stained with AO staining and TUNEL assay. Apoptosis, shown by white arrowheads, was detected around heart region by AO staining at 72 hpf (A) and 120 hpf (B). Apoptosis was also detected in heart area by TUNEL assay at 120 hpf (C). Quantitative analysis of apoptosis in AO staining was measured. Significant differences among groups were indicated by *p < 0.05 (D).
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