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Figure 6—figure supplement 2

ID: ZDB-IMAGE-210208-106
Source: Figures for Dilshat et al., 2021

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Figure Caption

Figure 6—figure supplement 2 MITF-KO cells mimic MRD in melanoma.

(a, b) Western blot analysis and quantification of MITF, ERK and p-ERK in EV-SkMel28, ΔMITF-X2, ΔMITF-X6 and miR-NTC, miR-MITF in SkMEL28 and 501Mel cell lines treated with DMSO or vemurafenib (1 µM) for 24 hr. Actin was used as loading control. (c, d) GSEA analysis using DEGs of ∆MITF-X6 vs. EV-SkMel28 cells on Rambow MRD and invasive gene signatures and MRD signature from Zebrafish and mitfa^low melanoma tumors. (e) Gene enrichment analysis plotted using Cluster profiler of single cell clusters obtained from melanoma tumors in zebrafish.

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