FIGURE 1

FIGURE 1

Nar protects against cisplatin ototoxicity. (A) Confocal image of a 5 d post‐fertilization (dpf) zebrafish. The neuromasts analysed in this experiment are indicated by black lines: SO1(supraorbital 1), O1 (otic 1), OC1(occipital 1), D1(dorsal trunk 1) and L1(lateral 1). Schematic top view of a 5 dpf neuromast showing the different cell types is shown in the right. (B) Animals were fixed and immunostained for MyosinVIIa (green) and DAPI. 5dpf zebrafish were incubated with 0, 150, 200 and 250 μmol/L cisplatin (CP) for 2 h, or pre‐treated with 0 µmol/L to 400 µmol/L of Naringin(Nar) for 3 h and then co‐treated with Nar and CP (150 µmol/L) for 2 h. (C) Diagram of the assay for B. (D and E) Quantification of the number of hair cells (MyosinVIIa⁺) per neuromast after the different treatments represented as mean ± SEM (n = 10). Control animals were exposed to vehicle alone (DMSO). ***P < 0.001. Black asterisks compared to DMSO‐treated animals. Red asterisks compared versus the corresponding CP concentration. (F) Scores for neuromast morphology of each group (The grading criteria are shown in the Materials and Methods). Scale bar in A equals 500 μm, B equals 5 μm