ZFIN Image: Khan et al., 2020, FIGURE 3

Image

Figure Caption

FIGURE 3

Carbofuran accelerated autolysosomal puncta formation in spns1 mutant zebrafish. A, Carbofuran treatment worsened the yolk opaqueness phenotype of spns1 mutant fishes, and most embryos lost their yolk extension. Loss of the spns1 gene increased autophagosomal EGFP‐LC3 and lysosomal LysoTracker (LysoT) red expressions, which were further accelerated by carbofuran exposure. Most LysoTracker red expressions were co‐localized with EGFP‐LC3 expressions (bottom row; yellow colour). B, Quantification of autophagosomal EGFP‐LC3 expression. C, Quantification of LysoTracker red expression. D, Quantification of the merged expression (co‐localization). These expressions were significantly increased by the loss of spns1 gene and were further significantly up‐regulated by carbofuran exposure. The scale bar is 10 mm; n = 6; *P ≤ .05; **P ≤ .01; ***P ≤ .005

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell. Mol. Med.