ZFIN Image: Guo et al., 2021, Figure 2

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Figure Caption

Figure 2

Effects of lhx4 knockdown via vivo-MO in the eyes on the ACs differentiation. All figures are horizontal sections along the temporal-nasal axis (T-N). (A–C’’) Immunofluorescence staining with different ACs markers, GABA, Parvalbumin, and Calretinin in WT, vCT, and 4vMO retinas at 84 hpf. Blue, DAPI staining of the nuclei. Scale bar = 20 μm. (a–c) Statistical analysis of GABA⁺, Parvalbumin⁺, and Calretinin⁺ cells in WT, vCT, and 4vMO retinas at 84 hpf. ns, P > 0.05; ****, P < 0.0001; vCT vs. WT; 4vMO vs. vCT. Results are presented as the mean ± SEM (n ≥ 10).

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