ZFIN Image: Chang et al., 2020, Figure 3

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Figure Caption

Figure 3

Effect of PMA on immortalized human keratinocyte (HACaT) cells. (A) Morphological image of HaCaT cells captured after 24 h of treatment with different concentration of PMA at different concentration (10 nM, 50 nM, 100 nM). Scale bar (100 μM), (B) HaCaT cell viability after 24 h PMA treatment, (C) LDH assay on HaCaT cells after 24 h PMA treatment, (D) H₂ DCFDA staining on HaCaT cells after 4 h PMA treatment (E) Hoechst staining on HaCaT cells after 4 h PMA treatment, (F) rhodamine 123 staining on HaCaT cells after 4 h PMA treatment, (100 μM), (G) IL-6 estimation on HaCaT cells, (H) TNF-α estimation, (I) IL-1β estimation, (J) Malondialdehyde assay, (K) Western blotting analysis. Densitometry analysis of these respective proteins were normalized by β-actin and evaluated through Image J software. The data are represented as mean ± S.D. of three independent experiments * p < 0.05, ** p < 0.01, *** p < 0.001 and ns (non-significant). Control vs. PMA. Statistical significance analysis was carried out through a one-way analysis of variance (ANOVA) prism.

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