ZFIN Image: Muchaamba et al., 2020, FIGURE 9

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Figure Caption

FIGURE 9

Impact of the PrfA truncation mutation predicted in N843_10 and N843_15 on virulence gene mRNA levels. Quantification of prfA, plcA, and hly mRNAs using qRT-PCR in the study strains that were cultured in BH broth at 37°C to the late exponential phase. Relative quantities (RQ) of prfA, plcA, and hly mRNA levels were normalized to 16S rRNA and are expressed relative to those of a L. monocytogenes EGDe based mRNA calibrator sample. Presented data shows the mean (bars) and standard deviation (error bars) of three independent biological experiments. ^∗Indicates statistically significant differences where P < 0.05 based on one-way ANOVA and Tukey post-hoc test pairwise comparison of all the strains.

Acknowledgments

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