Figure 5

Figure 5

RSCs require dnmt1 function to maintain proliferation. A–F Transverse sections of siblings (A–C) and dnmt1^−/− (D–F) larvae from 3 to 5dpf. Nuclei labeled with DAPI (gray). Cells in S-phase indicated by BrdU incorporation (magenta). Mitotic cells are labeled by a pH3(ser10) antibody (cyan). G Proportions of CMZ cells in S-phase (magenta), G2/M-phase (cyan), or not proliferating (gray) of both siblings and dnmt1^−/−larvae from 3 to 5dpf. H Proportion of CMZ cells labeled with BrdU from 3 to 5dpf between controls and dnmt1^−/−larvae. I Proportion of CMZ cells labeled with pH3 from 3 to 5dpf between controls and dnmt1^−/−larvae. White dotted lines designate CMZ (A–F). Scale bars: 30 μm *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.00005. Dorsal is up in all images.