ZFIN Image: Angileri et al., 2020, Figure 3

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Figure Caption

Figure 3

Loss of p53 function does not rescue the dnmt1^−/− CMZ phenotype. A–L Transverse sections of the dorsal CMZ in wildtype (A, E, I), dnmt1^+/+; p53^−/− (B, F, J), dnmt1^−/−; p53^+/+ (C, G, K), dnmt1^−/−; p53^−/− (D, H, L) larvae from 3 to 5dpf. Nuclei labeled with DAPI (gray) and F-actin labeled with phalloidin (magenta). M–P Graphs depicting changes in retinal domain proportions over time. Q–T Number of nuclei in each retinal domain of dnmt1^+/+; p53^+/+, dnmt1^+/+; p53^-/, dnmt1^−/−; p53^+/+, and dnmt1^−/−; p53^−/− larvae from 3 to 5dpf. GCL ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer; CMZ ciliary marginal zone. Scale bars = 25 μm. *p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.00005. Dorsal is up in all images.

Acknowledgments

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