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Figure 2
At low temperature, F507del‐zCftr generates a stable mature complex‐glycosylated protein. A, F508del‐hCFTR or F507del‐zCftr‐GFP was expressed in HEK293 cells. After 24 h incubation at 27°C, protein synthesis was inhibited by addition of CHX (0.5 mg/mL) and then the cells were shifted to 37°C to monitor thermal stability. Cells were collected after the indicated times for western blot analysis of whole cell extracts. B, The total amount of F508del‐hCFTR or F507del‐zCftr protein at each time point was quantitated and expressed relative to that at time 0 and calnexin loading (n = 4). CHX, cycloheximide; GFP, green fluorescent protein; hCFTR, human CFTR; HEK293, human embryonic kidney 293