ZFIN Image: Concilio et al., 2020, Fig 3

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Figure Caption

Fig 3 Flow cytometry characterization of transfected HEK293T cells and transduced HeLa cells expressing HA-human NIS, HA-minke whale NIS, and HA-zebrafish NIS.

(A) Percentage of cells positively stained with α-HA-AlexaFluor647 antibody; 24-hour post transfection HEK293T cells compared to puromycin selected lentivirally-transduced HeLa cells. (B) Mean fluorescence intensity of HEK293T transfected cells or transduced HeLa cells stained with α-HA-AlexaFluor647 antibody. (C) Percentage of puromycin selected lentivirally transduced HeLa cells over several passages which were stained with α-HA-AlexaFluor647 antibody. 3/T/P11 indicates cells which were frozen at passage 3 post-puromycin selection, thawed, and grown out to passage 11. (D) Median fluorescence intensity of puromycin selected lentivirally transduced HeLa cells over several passages which were stained with α-HA-AlexaFluor647 antibody. Values are averages of triplicate assays with standard deviation. -/+ Ab refers to incubation of antibody with nontransfected/untransduced parental cell line.

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