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Fig 2

ID
ZDB-IMAGE-200229-21
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Figures for Concilio et al., 2020
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Figure Caption

Fig 2 Characterization of HA-NIS protein expression and iodide transport.

Species are ordered in ascending evolutionary proximity to humans as determined by TimeTree (pig, mouse, rat, and dog diverged equidistantly) [73]. (A) Percentage of 1.25 μg/ml puromycin-selected cells which were positively-stained with α-HA-AlexaFluor647 antibody. (B) Median fluorescence intensity of 1.25 μg/ml puromycin-selected cells which were stained with α-HA-AlexaFluor647 antibody. Values are averages of duplicate assays. (C) Absolute uptake value of each NIS protein in the absence or presence of 100 μM perchlorate (ClO4), which has not been equalized to protein expression. Values are averages of triplicate assays with standard deviation. (D) Percentage of maximum 125I- uptake maintained in the presence of 100 μM perchlorate. Untrans. = untransduced, Ab = antibody.

Acknowledgments
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