IMAGE

Figure 7

ID
ZDB-IMAGE-200215-37
Genes
Source
Figures for Li et al., 2020
Image
Figure Caption

Figure 7 (A–D) Analysis of proliferative capacity of the adult intestine in celsr1a mutants and age matched wild-type fish (pulse injection and incorporation after 4 hr (red), nuclei counterstained by DAPI. (A, B) Low power view of comparable posterior regions of intestine of wild-type (A) and age and size matched frnt mutants (B). (C, D) Close up of intestinal rugae showing cells incorporating BrdU. (E–H), in situ hybridization of expression of sex-determining region Y-box 2 (sox2) (E, F) and olfactomedin 4 (olfm4). (G, H) genese in adult intestinal epithelia of wild-type (E, G) and celsr1a mutant (F, H) zebrafish. (I) Quantitation of changes in the number of olfm4+ cells observed in mutants; data presented as mean +/- standard deviation, *p<0.05, n = 5 (wt sibling), n = 7 (frnt) (J–K) Proliferative cells (24 hr after BrdU pulse, red) in comparison to celsr1a expression (green, yellow arrowhead) in larval developing intestine at 5dpf in (J) ceslr1aGFP heterozytotes and (K) ceslr1aGFP mutants; DAPI, blue. Insets J’ and J’’ and K’ and K’’are separate channels showing celsr1a expression and Brdu detection, respectively in the same tisse.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife