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Figure S4
HSPC Enrichments with Stained WKM Cells on BD FACSInflux, Related to
(A) Contour plots of stained WKM cells showing experimental sorting gates for HSPC for the WKM 11 experiment (representative example for WKM 11, 12 and 13 HSPC enrichment experiments) on BD FACSInflux. Sorted cells passed through gate 1 and gate 2. Percentages of events within each gate are indicated.
(B) t-SNE map of zebrafish WKM TD3 where HSPCs inside and outside of GateID gates are colored in red and blue respectively.
(C) Projection of the sorted GateID HSPCs for WKM 10 in FSC Height versus SSC Height (representative example for WKM 11, 12 and 13 HSPC enrichment experiments).
(D–F) Barplots and t-SNE maps showing the outcome of HSPC enrichments for (D) WKM 11, (E) WKM 12 and (F) WKM 13 on BD FACSInflux.
(G) Scatterplot showing experimental purities of GateID predicted gates determined by scRNA-seq (x axis) and histological analysis (y axis) for HSPCs (dark blue) and monocytes (light blue) on BD FACSJazz (triangle) and BD FACSInflux (circle).
(H) Design of
(I) Barplots indicating cell type percentages for the lymphocyte
Reprinted from Cell, 179, Baron, C.S., Barve, A., Muraro, M.J., van der Linden, R., Dharmadhikari, G., Lyubimova, A., de Koning, E.J.P., van Oudenaarden, A., Cell Type Purification by Single-Cell Transcriptome-Trained Sorting, 527-542.e19, Copyright (2019) with permission from Elsevier. Full text @ Cell