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Figure 4
GateID Allows Purification of Zebrafish Lymphocytes
(A) Contour plots of stained WKM cells showing experimental sorting gates for lymphocytes for the WKM 10 experiment (representative example for WKM 5, 6, 8, and 10 lymphocyte enrichment experiments) on BD FACSJazz. Sorted cells passed through gate 1 and gate 2. Percentages of events within each gate are indicated.
(B) Projection of the sorted GateID lymphocytes for WKM10 in FSC height versus SSC height (representative example for WKM 5, 6, 8, and 10 lymphocyte enrichment experiments).
(C) t-SNE map of zebrafish WKM TD2 where lymphocytes inside and outside of GateID gates are colored red and blue, respectively.
(D–G) Barplots and t-SNE maps showing the outcome of lymphocyte enrichments for (D) WKM 5, (E) WKM 6, (F) WKM 8, and (G) WKM 10 on BD FACSJazz.
See also
Reprinted from Cell, 179, Baron, C.S., Barve, A., Muraro, M.J., van der Linden, R., Dharmadhikari, G., Lyubimova, A., de Koning, E.J.P., van Oudenaarden, A., Cell Type Purification by Single-Cell Transcriptome-Trained Sorting, 527-542.e19, Copyright (2019) with permission from Elsevier. Full text @ Cell