ZFIN Image: Giallongo et al., 2019, Fig. 2

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Figure Caption

Fig. 2 PC activated MSC towards an inflammatory phenotype.

a Neutrophils isolated from co-culture with HS-5 or HC-MSC 24 h before pre-treatment with MM cells were able to inhibit T cell proliferation (b) and showed pro-angiogenic capacity in vitro. 1: HMEC control; 2: HMEC in presence of VEGF-A (positive control); 3: plus HC-MSCed-N; 4: plus HC-MSCed-N isolated from co-culture with HC-MSC pre-treated with MM cells. Magnification ×100. c Detection of NF-kB and IRF3 nuclear translocation was performed by incubation respectively with anti-mouse and anti-rabbit monoclonal antibodies followed by secondary antibodies conjugated to FITC (green) or TRITC (red). Counterstaining of cells was performed by using the nuclear dye, DAPI (blue). The photographs result from sequential analysis of the same microscopic field, followed by merging of different images with specific staining. *p < 0.05; **p < 0.01

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