ZFIN Image: Hsu et al., 2019, Fig. S8

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Figure Caption

Fig. S8

WT Cdk2 partially rescues neutrophil motility defects resulted from miR-199 overexpression.

(A) Schematics of experimental design. Embryos from Tg(lyzC:miR-199-Dendra2)pu19 were injected with a plasmid driving miR-199-resistant cdk2 expression in neutrophils, Tol2-lyzC-mCherry-2a-Cdk2 WT . Single (GFP+, miR-199 only) and double positive cells (GFP+mCherry+, miR-199+cdk2+) from the same embryos were tracked. (B, C) Representative images and tracks (B), and quantification of velocity (C) single and double positive cells. Scale bar: 100 μm. Green tracks: miR-199 expression only; Red tracks: miR-199 and cdk2 WT expression. 3 embryos each from three different founders were individual injected, imaged and quantified. Results are presented as mean ± s.d., Mann–Whitney test.

Acknowledgments

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