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Fig. S3
H149A mutant pETX fails to elicit dextran leakage or vascular pathology in hMAL-GFP expressing fish. Related to Figures 3 and 5. (A) 40X confocal images of hMAL-GFP expressing neurovasculature (green) co-injected with 2,000 kDa Dextran (red) and H149A pETX at 15MPI, 1 HPI, and 2HPI. First three columns represent 3-D volumes depicted in “Merge Macro” blue box. Scale bars= 30µm in first three columns and 50µm in “Merge Macro” column. (B) Example of 2HPI Imaris 3-D rendering of hMAL-GFP and Dextran surfaces. (C) hMAL-GFP expression and DIC confocal imaging of CCtA vessel at the three time points.
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