ZFIN Image: Elworthy et al., 2019, Fig. 10

Image

Figure Caption

Fig. 10

Endothelial‐specific excision of the Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488‐rescuing transgene causes no apparent further impairment to tagln‐expressing perivascular mural cells in ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488 fish. A: In situ hybridization for tagln expression on transverse posterior trunk frozen sections of 13‐dpf wt (wt) and sibling ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+ (ift54−/−;ubi:ift54) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+; Tg(kdrl:cre)s898^/+ (ift54−/−;ubi:ift54;kdrl:cre) fish. The red line in the cartoon illustrates where sections were taken. Tagln expression is detected at the site of arteries such as the dorsal aorta (arrow). The Tg(kdrl:cre)s898^/+ caused no apparent further impairment to perivascular mural cells in the fish with partial transgenic rescue of ift54^tp49. Images representative of four animals analyzed for each genotype. B: In situ hybridization for tagln expression on transverse caudal fin frozen sections of adult wt (wt) and sibling ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+ (ift54‐;ubi:ift54) and ift54^tp49; Tg(ubi:loxP‐ift54‐loxP‐myr‐mcherry,myl7:EGFP)sh488^/+; Tg(kdrl:cre)s898^/+ (ift54‐;ubi:ift54;kdrl:cre) fish. The red line in the cartoon illustrates where sections were taken. The Tg(kdrl:cre)s898^/+ caused no apparent further impairment to tagln expression in the fish with partial transgenic rescue of ift54^tp49. Images representative of four animals analyzed for each genotype. WT, wild‐type

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.