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Fig. 2

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Figures for Baek et al., 2019
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Fig. 2

Increased, Premature, and Prolonged LEC Specification in Nova2 Mutants

(A and B) Immunofluorescence (IF) staining of Prox1 at 28, 30, 32, 36, and 54 hpf in Tg(fli1a:nEGFP)y7 siblings and nova2uq14bh mutants. Arrowheads indicate Prox1-positive ECs (brackets indicate PCV). Left hand images show merged zoom out of trunk as an overlay of Prox1 and fli1a:nEGFP, which was used to confirm nuclear localization.

(C) Quantification of the number of Prox1-positive ECs at 28 hpf (siblings n = 14 and mutants n = 11), 30 hpf (siblings n = 23 and mutants n = 22), 32 hpf (siblings n = 18 and mutants n = 15), 36 hpf (siblings n = 40 and mutants n = 17), and 54 hpf (siblings n = 15 and mutants n = 15). For all stages, 7 body segments were quantified except at 54 hpf when 6 body segments were used (see also Figures S3A–S3E).

(D) Quantification of the number of Prox1-positive ECs in the PCV wall or having sprouted dorsally in 30 hpf sibling and mutant embryos. n = 7 Prox1-positive ECs in 4/23 embryos for siblings, and n = 33 Prox1-positive ECs in 14/22 embryos for mutants, with 11/33 Prox1-positive ECs sprouting from the PCV.

Error bars represent SD, Mann-Whitney-test (C), p < 0.05 ∗∗p < 0.01, and ∗∗∗p < 0.001; Scale bar represents 25 μm in (A) and (B).

See also Figure S3.

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Reprinted from Developmental Cell, 49, Baek, S., Oh, T.G., Secker, G., Sutton, D.L., Okuda, K.S., Paterson, S., Bower, N.I., Toubia, J., Koltowska, K., Capon, S.J., Baillie, G.J., Simons, C., Muscat, G.E.O., Lagendijk, A.K., Smith, K.A., Harvey, N.L., Hogan, B.M., The Alternative Splicing Regulator Nova2 Constrains Vascular Erk Signaling to Limit Specification of the Lymphatic Lineage, 279-292.e5, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell