ZFIN Image: Ding et al., 2019, Figure 2.

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Figure Caption

Figure 2. Whole organism imaging of PTA-stained zebrafish at cell resolution enables histology-like cross sections.

Coronal (A, H), sagittal (B, I), and axial (C–F, J–M) cross sections of 4 dpf larval (A–G) and 33-dpf juvenile (H–N) wild-type zebrafish acquired using synchrotron X-ray micro-tomography at 0.743 μm³ and 1.43 μm³ isotropic voxel resolution, respectively. 3D volume renderings (G, N) show the cross-sections in relation to the whole organism. In contrast to histology, the cross sections are a single voxel in thickness and can be obtained in any plane (including oblique cuts) after imaging. Complete cross-sections in the orthogonal directions for both fish are provided (Videos 2 and 3). Images are presented to match histological convention of dark cell nuclei (higher attenuation is darker).

Acknowledgments

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