ZFIN Image: Ding et al., 2019, Figure 4.

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Figure Caption

Figure 4. Comprehensive histological cross-sectioning of convoluted structures in juvenile zebrafish.

A 3D rendering of a whole juvenile (33 dpf) zebrafish is presented with highlighted gastrointestinal (GI) tract (A). The GI tract exemplifies a convoluted structure for that can be unraveled for histological analysis. The isolated GI tract is displayed (B). Isotropic resolution of data permits virtual slicing at any angle (B1–B3) without a decrease in resolution, allowing comprehensive histology-like visualization despite its tortuous nature. A spline-based reslicing method for visualizing serpentine organs across their entire path is also shown (C). A nonlinear cutting plane (blue) follows the structure of interest, allowing the cut to render a structure’s total length onto a single plane (D).

Acknowledgments

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