ZFIN Image: Hadjivasiliou et al., 2019, Figure 5

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Figure Caption

Figure 5

Transient Basal Protrusion Express Delta Protein, and Notch Activity Is Upregulated in Their Vicinity

(A and A′) DeltaD immunoreactivity (magenta) shows the localization of DeltaD aggregates in the basal protrusions and cell body of a T-shaped cell. The T-shaped cell expresses cytoplasmic GFP (green) under the DeltaD promotor.

(B) A T-shaped cell labeled with membrane-mKate (magenta) extends basal protrusions in a Tg(Tp1:VenusPEST) (green) embryo. The maximal extension of one basal protrusion is labeled with an arrow and dotted line. Squares indicate the two areas used for analysis of Tp1:VenusPEST expression.

(C) Graph showing the relative mean Tp1:VenusPEST fluorescence intensity under the basal protrusions compared to a control region outside the basal protrusions (unpaired one-tailed t test, p-value = 0.016, n = 13 basal protrusions (8 cells), the average (a intensity/ b intensity) is significantly greater than 1, mean ± SD = 7.2 ± 9.7).

Acknowledgments

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Reprinted from Developmental Cell, 49, Hadjivasiliou, Z., Moore, R.E., McIntosh, R., Galea, G.L., Clarke, J.D.W., Alexandre, P., Basal Protrusions Mediate Spatiotemporal Patterns of Spinal Neuron Differentiation, 907-919.e10, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell