Fig. 5.

Fig. 5.

PGE2 induces angiogenesis in zebrafish in a time-dependent manner. The dextran dye can trace cell lineage to track vascular development in whole fish. However as the embryo matures, the dye starts to diffuse, which makes it difficult to capture vascular maturation beyond 96 hpf. (A–F) The developing zebrafish trunk vasculature in both vehicle and PGE2 groups from 53 hpf to 96 hpf. The region of interest (ROI) in white dotted boxes shows the difference in vascular fluorescence between groups. (F) In this image, the ROI is expanded to show the dorsal aorta (DA), dorsal longitudinal anastomosing vessel (DLAV), posterior cardinal vein (PCV) and accompanying intersegmental arteries (ISA). (G) We selected a few zebrafish embryos [PGE2 (n=9) and vehicle (n=9)] in both groups to measure fluorescence intensity with ImageJ. The chart represents the mean fluorescence intensity±s.e.m. An unpaired t-test was conducted for each time point and we observed a significant increase in the trunk vasculature fluorescence in the PGE2 compared to the vehicle group at 72 hpf and 96 hpf, respectively. *P=0.007, **P=0.0001. Scale bar: 15 μm.