IMAGE

FIG 2 

ID
ZDB-IMAGE-190723-2172
Source
Figures for Willis et al., 2018
Image
Figure Caption

FIG 2 

Emergency granulopoiesis mediates long-term host defense. (A) Schematic of reinfection assays. At 2 dpf, wild-type (WT) AB zebrafish larvae were injected in the HBV with PBS or a low dose (0.5 × 103 to 2.0 × 103 CFU) of GFP+S. flexneri M90T. At 4 dpf, i.e., 48 h post-primary injection (hp1i), all larvae were injected with a high dose (>2.0 × 104 CFU) of mCherry-expressing (mCherry+) S. flexneri M90T. Analyses were performed on larvae up to 72 h post-secondary infection (hp2i). (B and C) WT AB larvae were injected with PBS (open circles) or “primed” with wild-type or T3SS GFP+S. flexneri M90T (closed circles), prior to a high dose of mCherry+S. flexneri M90T at 48 hpi, as described above. (B) Survival curves pooled from 4 independent experiments using n ≥ 9 larvae per condition per experiment. Up to three larvae per condition were taken for CFU at the 24 and 48 h time points. The top graph represents collated data. The bottom graph represents only Shigella-primed larvae, a subset of the above data. The P value between conditions was determined by log-rank Mantel-Cox test. Significance was defined as P < 0.05 (*). (C) Fluorescent mCherry+S. flexneri M90T burden of larvae was imaged by stereomicroscopy over time, and images were analyzed to produce fluorescence intensity measurements (as in Fig. S2A). Data were pooled from 4 independent experiments with n ≥ 4 per time point per condition per experiment. The top graph represents collated data. The bottom graph represents only Shigella-primed larvae, a subset of the above data. P values between conditions at cognate time points were determined by unpaired two-tailed Student’s t test. Significance was defined as P < 0.05 (*).

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ MBio