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Fig. 5-S1
Dorsalization induced by Overexpression of squint mRNA but not its non-protein coding version.
(A) Schematic representation of injected mRNAs. Cap-analog is indicated by in blue circles at the beginning of each mRNA. squint non-protein coding mRNA was generated by adding 8 Adenine-nucleotides (red circles) after in-frame ATG codons. (B) Table shows scoring outcome of observed phenotypes in embryos injected with 30 pg of each indicated mRNA. (C) Representative embryos showing typical wild-type, squint mutant or dorsalized morphology. Ambiguous phenotypes were scored as ‘Affected’.
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