ZFIN Image: Toselli et al., 2019, Fig. 2

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Figure Caption

Fig. 2

Inhibiting Vegf signaling does not affect beta-cell and alpha-cell formation. (a–c) Confocal projections of 72 hpf Tg(fli1:EGFP; insa:tagRFP) untreated, DMSO-treated, and SU5416-treated embryos from 12 to 72 hpf; endothelial cells (green), beta-cells (red), and DAPI nuclear stain (DNA; grey). Alpha-cells are labeled with a glucagon (GCG) antibody (blue). (d) The number of endothelial cells adjacent to beta-cells in untreated, DMSO-treated, and SU5416-treated embryos from 12 to 72 hpf. (e,f) The number of beta-cells and alpha-cells in Tg(fli1:EGFP; insa:tagRFP) untreated, DMSO-treated, and SU5416-treated embryos from 12 to 72 hpf. n = 14–20. (g) The number of beta-cells in Tg(fli1:EGFP; insa:tagRFP) untreated, DMSO-treated, and SU5416-treated embryos from 72 hpf to 92 hpf. n = 8–13. (h–j) Confocal projections of 96 hpf Tg(fli1:EGFP; insa:tagRFP) untreated, DMSO-treated, and SU5416-treated embryos from 72 to 96 hpf; endothelial cells (green), beta-cells (red), and DAPI (grey). (d–g) Box-and-whisker plots show median, and circles represent individual zebrafish. Scale bar = 10 μm.

Acknowledgments

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