ZFIN ID: ZDB-IMAGE-190628-60
Figures for Paatero et al., 2018

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Fig. 5

Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. ab Still images (Supplementary Movie 5) of an embryo showing the DLAV around 32 hpf in an embryo expressing both mRuby2-UCHD and VE-cad-Venus Tg(fli:Gal4ffubs3;UAS:mRuby2-UCHDubs20;BAC(cdh5:cdh5-Venus)). b A time series magnification of the inset in a. Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie 5) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD (Tg(fli:Gal4ffubs3;UAS:mRuby2-UCHDubs20;UAS:EGFP-hZO1ubs5)). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) (n = 7 embryos). f Close-up from panel e. Both channels are shown in inverted contrast. Scale bars 1 µm (bd) and 10 µm (ae)

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