ZFIN Image: Blokhina et al., 2019, Fig. 10

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Figure Caption

Fig. 10

spo11 -/- males do not produce sperm, while spo11 -/- females produce offspring that show a spectrum of abnormalities.

A: 1A-2A: Light microscope images of whole testes with spo11 -/- males showing a more translucent testis appearance as compared to WT. 3A-4C: Whole mount testes from WT and spo11 -/- males stained for Vasa (magenta) and DNA (DAPI, gray). Sperm clusters, indicated with "S" in WT, are absent in spo11 -/- mutants. B: Examples of offspring from spo11 -/- females showing the range of abnormalities at 28 hpf. hpf = hours post fertilization. C-D: Test crosses of spo11 -/- females (black circles) and WT control females (white circles) with WT males. Each circle represents a single test cross outcome. The data was pooled from two separate test crosses done on different days. The WT data at 5 dpf was assessed from one of the test crosses but was not tracked up to 5 days for the other WT test cross. C: spo11 -/- females produced similar numbers of eggs to WT females. D: The percent of eggs that were fertilized, and the percent of offspring that were normal or near normal in their development at 1 dpf, and at 5dpf. dpf = days post fertilization.

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Acknowledgments

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