ZFIN Image: Guner-Ataman et al., 2018, Fig. 2

Image

Figure Caption

Fig. 2

tbx1 Mutant Embryos Lack nkx2.5⁺ Pharyngeal Progenitors

(A and B) Merged bright-field and fluorescent images of 32 hr post fertilization (hpf) control (CTRL; A; n = 30) and tbx1 mutant (B; n = 8) embryos carrying the Tg(nkx2.5:ZsYellow) transgene. Dorsal views, anterior up. The numbers in (A) identify each pharyngeal cluster.

(C and D) Bright-field z-stacks of 32 hpf CTRL (C; n = 51) and tbx1 mutant (D; n = 18) embryos processed by in situ hybridization with an nkx2.5 riboprobe. The numbers in (C) identify each pharyngeal cluster. Dorsal views, anterior up.

(E and F) Merged bright-field and fluorescent images of 20 hpf CTRL (E; n = 30) and tbx1 mutant (F; n = 8) embryos carrying the Tg(nkx2.5:ZsYellow) transgene. The brackets in (E) highlight the ZsYellow⁺ the pharyngeal progenitor population missing in (F). Dorsal views, anterior up.

(G and H) Bright-field z-stacks of 20 hpf CTRL (G; n = 32) and tbx1 mutant (H; n = 10) embryos processed by in situ hybridization with an nkx2.5 riboprobe. The brackets in (G) highlight the nkx2.5⁺ pharyngeal progenitor population missing in (H). Dorsal views, anterior up. In all cases, little to no variation was observed between animals in each experimental group.

Scale bars, 25 μm. H, heart.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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