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Fig. 3
CR, CB and PV expression in neurons with identified neurotransmitter type. a–c Double immunostaining experiments for CR, CB and PV (magenta) with markers that label the GABAergic, glycinergic, glutamatergic, cholinergic and serotonergic spinal neurons (green) in whole mount adult zebrafish spinal preparations. Enlarged and single channel views are given for areas indicated by framed boxes. Arrowheads indicate double-labeled cells. d–f Quantification of the percentage of CR, CB and PV positive neurons that localized in neurons with a specific neurotransmitter phenotype
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