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Fig. 6 JNK is activated by Nodal from the YSL. (A) Transverse sections of DMSO-treated control and SB505124-treated embryos; p-JNK and F-actin were visualized by immunohistochemical staining and phalloidin, respectively at 4.7 hpf. Fluorescent signals are displayed as grayscale images. Arrowheads indicate p-JNK fluorescence on the boundary between the YSL and LMCs. Asterisk indicates the cell membrane of EVL cells. Scale bars: 10 µm. (B) Fluorescence intensities were measured along the cell membrane on the boundaries between the YSL and LMCs (V), on cells located animal-pole side and LMCs (A), EVL and LMCs (L), or MMCs and LMCs (M). Error bars represent s.d. of ten measurements. n, number of nuclei examined. ***P<0.001.