ZFIN Image: Mueller et al., 2017, Fig. 2

Image

Figure Caption

Fig. 2

Cytoskeletal Response to Manipulations of Membrane Tension

(A) Lifeact:GFP imaging following micropipette aspiration. (1) Migrating keratocyte before contact with the micromanipulator, (2) aspiration of membrane, and (3) release of vacuum.

(B) Temporal fluctuations of lifeact:GFP intensity at the leading edge defined as in Figure 1B, area, and protrusion speed. The area between the dotted lines shows where the cell was aspirated.

(C) Kymograph along dashed yellow line in (A).

(D) Change in lifeact:GFP signal following aspiration with four different vacuum levels.

(E) Change in cell edge protrusion for the same cells as in (D). Mean and SEM are shown for 28 aspiration events in 13 individual cells in (D) and (E).

(F) Lifeact:GFP signal following fast cell shrinkage. (1) Keratocyte exhibiting stretched morphology, while its trailing edge is tethered to the substrate. (2) The trailing edge is cut with a pulsed laser (yellow arrowhead).

(G) Temporal fluctuations of leading edge lifeact:GFP intensity, area, and protrusion speed. The rapid retraction is marked with a dotted line.

(H) Kymograph along dashed yellow line in (F).

See also Figure S3 and Movie S2.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 171, Mueller, J., Szep, G., Nemethova, M., de Vries, I., Lieber, A.D., Winkler, C., Kruse, K., Small, J.V., Schmeiser, C., Keren, K., Hauschild, R., Sixt, M., Load Adaptation of Lamellipodial Actin Networks, 188-200.e16, Copyright (2017) with permission from Elsevier. Full text @ Cell