IMAGE

Fig. 5

ID
ZDB-IMAGE-180118-25
Genes
Source
Figures for Bayramli et al., 2017
Image
Figure Caption

Fig. 5

Functional equivalence and bias in OSN neurogenesis at the ILC and SNS. (A) In situ-hybridization (red) for markers of distinct OSN subtypes and immunohistochemistry against BrdU (blue) at 8d (trpc2, ompb) and 4d (v2rl1, or112-1) following 24 h BrdU incubation. The trpc2 gene is expressed by all microvillous OSNs, a larger subset of which also expresses the vomeronasal receptor v2rl1. Ciliated OSNs label positive for ompb and express specific olfactory receptor genes, such as or112-1. For each gene, double-positive cells (arrowheads) can be found at the interlamellar curves (asterisks) and the sensory/nonsensory border (dotted line). The solid line demarcates the outline of the section of which only one half is shown. Scale bars: 100 µm. (B) Quantification of OSN subtype neurogenesis at different proliferation sites. The sensory OE was subdivided into four equal segments (ILC: interlamellar curves, Sc: sensory central, Sp: sensory peripheral, SNS: sensory/nonsensory border) and the occurrence of marker/BrdU double-positive OSNs was scored for each segment for the general microvillous marker trpc2 (red), the vomeronasal-like receptor v2rl1 (light red), the ciliated OSN marker ompb (blue) and the three classical OR genes or101-1, or107-1, and or112-1 (light blue) expressed by ciliated OSNs. A subtype-specific bias exists for neurogenesis at the ILC and SNS. Data represent the mean ± SEM; trpc2: 607 cells from 5 OE; v2rl1: 203 cells from 4 OE; ompb: 563 cells from 2 OE; or101-1: 35 cells from 6 OE; or107: 25 cells from 7 OE; and or112-1: 76 cells from 8 OE.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.