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Fig. 6
Actomyosin dynamics and protrusive activity during convergence and lateral cell movements. a Schematic view of cells imaged in b, c. Wild-type embryos were transplanted with cells from a ßactin:myosinII-GFP transgenic donor injected with mbCherry mRNA. b Anterior OP cells undergoing convergence movements towards the placode centre. Dynamic accumulations of myosin II can be observed in the cell bodies, in the front or back of moving cells (white arrows). The yellow arrow indicates myosin II accumulation during ring contraction after a cell division. c Central OP cells undergoing lateral movements. No myosin II can be detected in the cell bodies. Myosin II rather accumulates at the tip of the axonal protrusions (arrows). Coloured dots indicate the cell bodies of cells of interest. d Schematic view of cells imaged in e, f. Wild-type embryos were transplanted with cells from a donor embryo expressing Utrophin-GFP (actin probe) and mbCherry. e Anterior OP cells converging along the brain surface exhibit canonical morphologies of actively migrating cells, with dynamic filopodia (white arrowheads) and actin accumulation at their leading edge. Green arrows indicate protrusions that do not belong to the two cells of interest, but to a more anterior cell following them. f In cells moving laterally, filopodia and actin are observed at the tip of axons, but not at the level of the cell bodies. Scale bars: 10 µm