ZFIN Image: Eum et al., 2016, Fig. 2

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Fig. 2

Trinitrotoluene (TNT) in pink water causes defects in heart development and function. (A) Tg(cmlc2:EGFP) embryos treated with pink water have myocardiac defects. Embryonic myocardiocytes were imaged at 38 hpf (hours post fertilization) with heads orientated upwards, scale bar 500 µm. Myocardiocytes in the atrium and ventricle were visualized by enhanced green fluorescent protein (EGFP) fluorescence in controls (a) and in embryos treated with pink water containing 13.5 µg/mL TNT for 33 h (b); (B) Heart rate is dose-dependently decreased in 38 hpf embryos by exposure to pink water containing TNT at 0, 0.27, 1.35, 2.70 and 13.5 µg/mL (n = 20 each). Following exposure to pink water from 5 hpf, Tg(cmlc2:EGFP) embryonic hearts were imaged laterally at 38 hpf using an Axioimager II fluorescence microscope (Carl Zeiss, Overkochen, Germany). * p < 0.05, ** p < 0.001.

Acknowledgments

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