IMAGE

Fig. 3

ID
ZDB-IMAGE-160728-35
Source
Figures for Zhong et al., 2014
Image
Figure Caption

Fig. 3

zNoxa controls mitosis and apoptosis during early embryogenesis. (a) Confocal z-stacks of apoptotic cells assessed by TUNEL in embryos which were WT (i, v) and injected with zNoxa MO1+2 (ii, vi), zNoxa mRNA (iii, vii) or zNoxa mismatch MO1+2 (iv, viii) at 75% epiboly and bud stages. Embryos were lateral views with animal pole toward the top and dorsal to the right. (b) Quantification of numbers of TUNEL-positive cells with approximately four embryos for each group in a. Error bars represent S.E.M. (n=4). (c) Confocal z-stacks of mitotic cells assessed by pH3 (phosphorylated histone H3) staining in embryos which were WT (i, vi) and injected with zNoxa MO1+2 (ii, vii), zNoxa mRNA (iii, viii), combined zNoxa MO1+2 and rcRNA (iv, ix) or zNoxa mismatch MO1+2 (v, x) at shield and 75% epiboly stages. Embryos stained by pH3 were lateral views with animal pole toward the top and dorsal to the right. (d) Quantification of numbers of pH3-positive cells with five embryos for each group in c. Error bars represent S.E.M. (n=5 embryos each). (e) A typical FACS analysis of PI (propidium iodide)-labeled cells in embryos for each group in c. (f) The FACS statistical data of three independent experiments in e. Error bars represent S.E.M. (n=3). (g) Confocal z-stacks of mitotic cells assessed by pH3 staining in embryos which were injected with zNoxa MO1+2 plus mNoxa mRNA or mNoxa mRNA at shield stage. (h) Quantification of numbers of pH3-positive cells with five embryos for each group in g. Error bars represent S.E.M. (n=5 embryos each). (i) A typical FACS analysis of PI-labeled cells in embryos for each group in g. (j) The FACS statistical data of three independent experiments in i. Error bars represent S.E.M. (n=3)

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Differ.