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Fig. S7
γH2AX immunoreactivity in UV-irradiated zebrafish tissue or zebrafish photoreceptors after MNU treatment. A. Whole-mount immunofluorescence for γH2AX in UV -irradiated zebrafish embryos. Zebrafish embryos at 24 h post fertilization were irradiated to 2.43 J/cm2 ofUV-C by using UVGL-58 handheld UV lamp (UVP, Upland, CA, USA). Embryos were then cultured in a dark incubation chamber for 30 min at 28.5°C and fIXed with 4% paraforrn-aldehyde for 1 h at 4°C. Whole-mount immunofluorescence was as previously described by Komoike and Matsuoka (2013). Scale bar = 100 µm. B. Immunofluorescence for γH2AX in UV -irradiated zebrafish embryos using cryostat sections. Scale bar =20 µm. C. Double immunofluorescence for BrdU and γH2AX in the zebrafish retinas after MNU treatment. Photoreceptors in the outer nuclear layer (ONL) are γH2AX+ at day I after treatment while BrdU+ cells at later time points are negative for γH2AX. INL, inner nuclear layer; Scale bar = 20 µm.