IMAGE

Fig. S1

ID
ZDB-IMAGE-160205-102
Genes
Source
Figures for Khaliq et al., 2015
Image
Figure Caption

Fig. S1

id2a mRNA, but not tp53 MO, injection rescues the reduced liver size in id2a MO-injected embryos. (A, B) Epifluorescence images showing fabp10a:dsRed expression (red) in control, id2a MO-injected, and id2a MO + mRNA-co-injected embryos (A) and their quantification (B). Although the liver of the co-injected embryos was still smaller than the control liver, it was much larger than the liver of the single MO-injected embryos, indicating a partial rescue of the liver size defect exhibited in id2a MO-injected embryos. For quantification, embryos were divided into three groups based on liver size: small, medium, and large. The id2a MO-injected liver size shown in A was considered as small; the liver size of the co-injected embryo shown in A was considered as large. Dorsal view, anterior to the left. (C) Bright-field and epifluorescence images showing the overall morphology of embryos and fabp10a:dsRed expression (red), respectively, in control, tp53 MO-injected, id2a MO-injected, and id2a MO + tp53 MO-co-injected embryos. Liver size as well as eye and head size (arrows) in id2a MO-injected embryos was similar to that in embryos co-injected withid2a and tp53 MOs. (D) Quantification of the results in C. For quantification, the liver size of the control embryo shown in C was considered as large and the liver size of the id2a MO-injected embryo shown in C was considered as small. Lateral view, anterior to the left. (E) Epifluorescence images showing the fabp10a:dsRed expression (red) in control and id2a mRNA-injected embryos at 72 hpf. Liver size in id2a-mRNA injected embryos was similar to that of control embryos. Lateral view, anterior to the left. Scale bars: 100 µm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 138 Pt 3, Khaliq, M., Choi, T.Y., So, J., Shin, D., Id2a is required for hepatic outgrowth during liver development in zebrafish, 399-414, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.