ZFIN ID: ZDB-FIG-160205-115
Khaliq et al., 2015 - Id2a is required for hepatic outgrowth during liver development in zebrafish. Mechanisms of Development   138 Pt 3:399-414 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Pec-fin to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Pec-fin to Protruding-mouth

Fig. S1

id2a mRNA, but not tp53 MO, injection rescues the reduced liver size in id2a MO-injected embryos. (A, B) Epifluorescence images showing fabp10a:dsRed expression (red) in control, id2a MO-injected, and id2a MO + mRNA-co-injected embryos (A) and their quantification (B). Although the liver of the co-injected embryos was still smaller than the control liver, it was much larger than the liver of the single MO-injected embryos, indicating a partial rescue of the liver size defect exhibited in id2a MO-injected embryos. For quantification, embryos were divided into three groups based on liver size: small, medium, and large. The id2a MO-injected liver size shown in A was considered as small; the liver size of the co-injected embryo shown in A was considered as large. Dorsal view, anterior to the left. (C) Bright-field and epifluorescence images showing the overall morphology of embryos and fabp10a:dsRed expression (red), respectively, in control, tp53 MO-injected, id2a MO-injected, and id2a MO + tp53 MO-co-injected embryos. Liver size as well as eye and head size (arrows) in id2a MO-injected embryos was similar to that in embryos co-injected withid2a and tp53 MOs. (D) Quantification of the results in C. For quantification, the liver size of the control embryo shown in C was considered as large and the liver size of the id2a MO-injected embryo shown in C was considered as small. Lateral view, anterior to the left. (E) Epifluorescence images showing the fabp10a:dsRed expression (red) in control and id2a mRNA-injected embryos at 72 hpf. Liver size in id2a-mRNA injected embryos was similar to that of control embryos. Lateral view, anterior to the left. Scale bars: 100 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed gz15Tg control Pec-fin liver IHC
Protruding-mouth liver IHC
gz15Tg + MO1-id2a standard conditions Pec-fin liver IHC
Protruding-mouth liver IHC
gz15Tg + MO1-id2a + MO4-tp53 standard conditions Pec-fin liver IHC
gz15Tg + MO4-tp53 standard conditions Pec-fin liver IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gz15Tg + MO1-id2a standard conditions Pec-fin liver decreased size, abnormal
Pec-fin liver DsRed expression decreased amount, abnormal
Protruding-mouth liver decreased size, abnormal
Protruding-mouth liver DsRed expression decreased amount, abnormal
gz15Tg + MO1-id2a + MO4-tp53 standard conditions Pec-fin liver decreased size, abnormal
Pec-fin liver DsRed expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 138 Pt 3, Khaliq, M., Choi, T.Y., So, J., Shin, D., Id2a is required for hepatic outgrowth during liver development in zebrafish, 399-414, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.