ZFIN Image: Ariotti et al., 2015, Fig. 2

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Figure Caption

Fig. 2

APEX-GBP Allows for Time-Resolved Protein Redistribution

(A–F) BHK cells were transfected and serum starved for (A) 0 min, (B) 30 min, (C) 1 hr, (D) 2 hr, (E) 4 hr, and (F) 8 hr to track the redistribution of GFP-Cav^DGV by APEX-GBP in a time-resolved manner. Arrows highlight regions where electron density has re-distributed from lipid droplets to other intracellular organelles. LD, lipid droplet; Mt, mitochondria; EE, early endosome; LE, late endosome; ER, endoplasmic reticulum; Nuc, Nucleus; Cav, Caveolae. Scale bars represent 500 nm. The above localizations were confirmed by confocal microscopy; see Figure S2.

Acknowledgments

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Reprinted from Developmental Cell, 35(4), Ariotti, N., Hall, T.E., Rae, J., Ferguson, C., McMahon, K.A., Martel, N., Webb, R.E., Webb, R.I., Teasdale, R.D., Parton, R.G., Modular Detection of GFP-Labeled Proteins for Rapid Screening by Electron Microscopy in Cells and Organisms, 513-25, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell