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Fig. 7

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ZDB-IMAGE-151113-9
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Figures for Hoeppner et al., 2015
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Fig. 7

RhoC negatively regulates VEGF-induced vascular permeability in zebrafish. (A,B) Using zebrafish cDNA, a probe to zebrafish RhoC (Rhoad) was created and in situ hybridization was performed on 24hpf zebrafish embryos. Multiple images were captured using a Zeiss Axioplan 2 microscope and overlayed in Photoshop, such that areas of focus were unmasked, to generate a composite image. Lateral (A) and superior (B) views are shown. DA, dorsal aorta; PCV, posterior cardinal vein; ISVs, anterior intersomitic vessels; NT, neural tubes. (C) Microangiography was performed on anesthetized 3dpf zebrafish embryos by injecting FITC–dextran (2000kDa) and Texas-Red–dextran (70kDa), VEGF was induced through heat exposure (when applicable), and extravasation of red tracer as a measure of zebrafish vascular permeability was live imaged using a ZEISS LSM 780 confocal microscope. Control, no MO injection and no VEGF induction; Cont MO, control MO injection; RhoC MO, Rhoad and Rhoae MO injection; Unind, no VEGF induction; VEGF, heat induction of VEGF transgene. (D) Quantification of extravasated red tracer. **P<0.05 (RhoC MO, VEGF Induced versus Control MO, VEGF Induced); *P<0.05 (RhoC MO, Uninduced versus Control; Control MO, VEGF Induced versus Control) (paired two-tailed Student′s t-test). Results are mean±s.d. (n=??).

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.