IMAGE

Fig. S2

ID
ZDB-IMAGE-151008-23
Source
Figures for Hu et al., 2015
Image
Figure Caption

Fig. S2

Evaluation of the knockdown efficiency of ddb1 morpholinos.

(A-B) RT-PCR to test the efficiency of ddb1 splice site targeted morpholino (ddb1_MO1). (A) Schematic diagram of ddb1_MO1 targeting the 5th exon-intron junction. (B) Efficiency assay of ddb1_MO1 by RT-PCR using two pairs of ddb1-specific primers. The amount of RNA and cDNA used for RT-PCR was the same for the different samples and for the internal control β-actin. Negative control (con) contained no cDNA. (C-D) Evaluation of the knockdown efficiency of ddb1 translation start site morpholino (ddb1_MO2). (C) Schematic representation of ddb1-egfp fusion construct used for ddb1_MO2 efficiency assay. (D) The expression of EGFP in larvae injected with ddb1-egfp fusion construct together with or without ddb1_MO2. Anterior towards the left. Abbreviations used: E4/E5/E6, 4th /5th /6th exon; I4/I5/I6, 4th / 5th / 6th intron; ddb1_LF/R, the forward and reverse primers of longer ddb1-specific fragment; ddb1_SF/R, forward and reverse primers of shorter ddb1-specific fragment; 1 and 2, different samples; uninj, uninjected embryos.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One