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Fig. S1 Formation of primary retinoblastoma, invasion and metastasis of non-invasive retinoblastoma cells in the zebrafish.
Approximately 150 DiI-labeled human non-invasive WERI-Rb1 retinoblastoma cells (red color) were intravitreally implanted into the eye of zebrafish embryo. The formation of primary tumors, and invasion and metastasis were kinetically monitored under fluorescent microscopy at different time points after tumor cell implantation. Approximately 30-40 zebrafish were used for this experiment.
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