|
Fig. 9
Overexpression of miR-19b altered lft2, foxj1a and gata4 expression. Representative images of lft2 expression in the cardiac field are shown using a dorsal view of 22-so-mite-stage embryos, with the anterior end at the top. The expression position of lft2 was altered in miR-19b mimic-injected embryos (C), compared with WT (A) and NC-injected (B) embryos. Representative images of foxj1a expression in DFCs are also shown. Foxj1a expression in DFCs was severely downregulated in miR-19b-overexpressing zebrafish embryos (F), compared with WT (D) and NC-injected (E) embryos. Representative images of gata4 expression are presented. Gata4 expression was restricted to the rostral Anterior lateral plate mesoderm (ALPM) in WT embryos (G) and in embryos injected with NC (H); however, its expression was expanded caudally and laterally in embryos injected with miR-19b (I). The asterisk indicates alteration of gata4 expression.