|ZFIN ID: ZDB-IMAGE-141001-4|
(a1–d1) DCFH-DA reveals the gut lumen formation process at 2–4.5 dpf in the lateral view. (a2–d2) The dorsal view of the pattern of a1–d1 at 2–4.5 dpf. The red arrows in a1–d2 represent the intestinal lumen formation processes, which initially show a dashed line pattern (boxed region in a1–b1, red arrows in a2) at 2 dpf and merge thereafter. The red arrowheads in a1 to d2 indicate the formation of the intestine bulb from 2 dpf, which increases in size at 2.5 dpf (b1 and b2), 3.5 dpf (c1 and c2) and 4.5 dpf (d1 and d2). (e1–f4) The staining patterns of DCFH-DA at later stages, 5 dpf (e1–e4) and 6 dpf (f1–f4). e1–e2 are lateral with regard to the gut after staining, and e2 is the image of e1 merged with DIC. The blue arrows in e1 and e2 indicate that the dye marks the pronephric ducts in addition to the gut lumen, as indicated by red arrows. e3–e4 shows the dorsal view of the pattern, which indicates that the dye clearly labels the gallbladder (white arrows). e4 is the image of e3 merged with DIC. f1–f4 are the lateral views of the gut at 6 dpf, and f2 and f4 are the images of f1 and f3 merged with DIC. f3 and f4 are high magnifications of the boxed images in f1 and f2. The white arrowheads in f3 and f4 indicate the folding of the gut epithelium during the formation of crypt-like architecture. (g–h) The dye emitting from the mouth (g) and anus (h). The red arrows represent the circular signals of the emitting dye under the GFP channel.
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