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Fig. S2 Random activation of oncogenic H-RAS by stochastic Gal4 expression. Larvae were heat shocked (HS) at various time points, as indicated. Larvae were derived from a cross Tg(hsp70:mCherry-G23-Gal4VP16)hu7161 with Tg(UAS:EGFP-H-RAS_G12V)io6. Larvae subjected to heat shock treatment showed stochastic expression of EGFP-H-RAS(G12V), hyperpigmentation and tumor formation. The level of these phenotypes was depended on the number of heat shock treatments (see Table 1 for quantification). Images were taken at 126 hpf. Scalebar indicates 0.5 mm.
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Full text @ Dis. Model. Mech.