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Fig. 4 Stochastic activation of Gal4-VP16. (A) (Upper panel) Schematic representation of plm75 in which the coding sequence of Gal4-VP16 is placed downstream of that of mCherry, but out-of-frame because of a G23 intervening sequence, all under the control of the heat shock hsp70 promoter. (B) A representative (merged) image shows stochastic expression of Kaede in an embryo at 5 dpf from a cross of the transgenic line Tg(hsp70:mCherry-G23-Gal4-VP16)hu7161 with Tg(UAS:Kaede). (C) Image of a single neuron that was activated stochastically (indicated with white arrows).
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Full text @ Dis. Model. Mech.