Fig. S2

Fig. S2

Treatment with a calcineurin inhibitor does not result in disproportionate growth between tissues, related to Figure 1. (A) Masson’s Trichrome-stained cross section through a bony ray of a DMSO-treated 7 dpa regenerating fin. (B) Masson’s Trichromestained cross section through a bony ray of a FK506-treated 7 dpa regenerating fin. (C) Higher magnification of Toluene blue-stained section of a distal regenerating tip of DMSOtreated fins at 7 dpa. (D) Higher magnification of Toluene blue-stained section of a distal regeneration tip of FK506-treated fins at 7 dpa. (E) Graphed measurements of the epidermal thickness (width) from bone to outer surfaces at the 0, 100 and 200 µm from the distal tip of DMSO- and FK506-treated regenerating fins at 7 dpa. (F) Graph of the thickness (width) of the central mesenchymal compartment between bone hemirays at 100 and 200 μm from the distal tips of DMSO- and FK506-treated regenerating fins at 7 dpa. (G) Graph of area measurements of several cells (n = 6) in the wound epidermis and blastema of DMSO- and FK506-treated fins. (H) Graph of the number of cells from the distal tip to 200 μm in the wound epidermis and blastema of DMSO-and FK506-treated fins. (I) BrdU-incorporation (red) in DAPI-stained (blue) fin treated with DMSO for 42 days. (J) Cross section of BrdU– labeled (red) in DAPI-stained (blue) fin. (K) BrdU-incorporation (red) in DAPI-stained (blue) fin treated with FK506 for 42 days. (L) Cross section of BrdU-labeled (red) in DAPI-stained (blue) fin. (M) Graph of BrdU measurements of BrdU-positive nuclei observed from cross sections. (n = 3 caudal fins per treatment group, and 3 different section locations per fin). p values determined by Students’s T-test. Scale bars are 2 mm (A,B); 400 μm (C,D) (I,K) 100 μm and (J,L) 20 μm. (E-H,M) Data are represented as mean +/- SD.