|
Fig. S1
FK506 treatment enhances regenerative growth of fins, related to Figure 1. (A,B) Regenerated adult DMSO-treated (A) and FK506-treated (B) caudal fins at 28 dpa showing the points of reference (base of fin and amputation plane) used to determine changes in length proximal to the amputation plane (determined by change in bone morphology and thickness of pigmentation stripes at the amputation border between the residual stump and regenerated tissue, black arrowhead). (C) Graph of measurements from different fish (n = 6) per treatment group. (D) Regenerating anal fin of adult fish 28 days post DMSO treatment and amputation. (E) Regenerating anal fin of adult fish treated 28 days post FK506 treatment and amputation. (F) Regenerating dorsal fin of adult fish 28 days post treatment with DMSO. (G) Regenerating dorsal fin of adult fish 28 days post treatment with FK506. (H) Graph of fin-to-body ratios of anal and dorsal fins from both treatments (n = 6 per treatment group). All graphs show mean +/- SD. p values determined by Students’s T-test. Arrowheads indicated plane of amputation. (A-G) Scale bars equal 1mm (A,B) and 2 mm (D-G).
Reprinted from Developmental Cell, 28, Kujawski, S., Lin, W., Kitte, F., Börmel, M., Fuchs, S., Arulmozhivarman, G., Vogt, S., Theil, D., Zhang, Y., Antos, C.L., Calcineurin regulates coordinated outgrowth of zebrafish regenerating fins, 573-87, Copyright (2014) with permission from Elsevier. Full text @ Dev. Cell